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Two-colour nanometre-scale resolution imaging by Minimal Photon Fluxes (MINFLUX), an ultra-high resolution microscopy technique that combines aspects of single-molecule localisation microscopy and stimulated emission depletion (STED) microscopy 14 , was employed to determine the average distance between endosomal SNAP/FLAG-hGLP-1R and VAPB, estimated at around 28 nm (Fig

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