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For permeabilisation, sections were incubated in 0.1% Triton X-100 (in PBS) for 20 min, blocked for 1 h in 5% normal goat serum, and then incubated overnight at 4 C with primary antibody: GLP-1R (MAB7F38, DSHB) diluted 1:30 in 1% BSA/PBS

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