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The infected mice exhibited a higher increase in interleukin (IL) IL-5, IL-4, and IL-2 than IL-12 and interferon- (IFN-), suggesting an important alteration in cytokine profile from Th1 to Th2 (Figure 2) [38]
High doses dont necessarily speed up healing and may waste product or desensitize receptors over time
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The messenger RNA (mRNA) expression levels of the target genes were determined by the 2 Ct method, using the following formula: [ Ct = (Ct gene of interest Ct actin ) treated (Ct gene of interest - Ct actin ) untreated ] (Li et al., 2015)
This guide is built around the part that matters most in real lab handling
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