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Plates were incubated at RT for 60 min before measuring the fluorescence intensity at 620 nm and 650 nm by an EnVision multilabel plate reader (PerkinElmer, Boston, USA)

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The micro-step approach, visible on the plotter as a gentler incline rather than sharp staircase, keeps transition spikes below 10% of steady-state peaks

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I think people see that about us, and they're drawn to it as well."