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no standard dose reduction is required
Primary hepatocytes were infected with Ad-TetOn-NHK or Ad-CMV-eGFP at a multiplicity of infection of 1:1
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The substrate acetylthiocholine iodide is then added to produce thiocholine which in the presence of oxidizing agent 5,5-dithio-bis-2-nitrobenzoic acid (DTNB) generates a yellow colour, which was measured at 405 nm
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