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Methodologically, these assays utilize workflows adapted from tissue/mucosal SAAs (e.g., careful avoidance of surface contamination and the use of matrix-matched controls)

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Functionally, cagrilintide activates amylin receptors AMY1R, AMY2R, AMY3R and the calcitonin receptor (CTR), binding via a conserved bypass motif involving mid-segment residues (S19 P25) (Cao et al

Funding This work was supported by the Heart and Stroke Foundation of Canada (HSFC Grant in Aid Award #G-14-0005706 and HSFC-Ontario Personnel Award Phase I Career #7500 to AMJ), South Eastern Ontario Academic Medical Organization (SEAMO Clinician Award to AMJ), and Queens University (Department of Medicine Research Award to AMJ)

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IGF-1 LR3 is a synthetic analog of insulin-like growth factor-1 (IGF-1) modified with an arginine substitution at position 3 and an extended 13-amino-acid N-terminal sequence