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GIPR-Camper neurons of the AP were excited using a light-emitting diode (LED) light source (Dual Optoled, Cairn Research) at 435 nm for 50 ms every 3 s, and CFP and YFP emissions were simultaneously recorded after passing through an optosplit (Cairn Research
A., Heisel, W
The adducts were screened by the sitting-drop vapour diffusion method using commercially available screens from Molecular Dimensions and Qiagen, dispensed by the Mosquito robot (TTP Labtech)
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Always use bacteriostatic water which keeps peptide stable for 28 days
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