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Gut-Flora-Dependent metabolite trimethylamine-N-Oxide promotes atherosclerosis-associated inflammation responses by indirect ROS stimulation and signaling involving AMPK and SIRT1

For best results, combine with regular physical activity and a low-fat diet

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Total protein of the cells was extracted and reduced in the presence of 100 mmol/L DTT for 5 min on the boiling water bath, and subsequently alkylated with 50 mol/L iodoacetamide for 30 min at room temperature in the dark

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When eating more processed foods, they ate faster and gained about two pounds over a two week period