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doi: 10.1128/mSystems.00169-16 52

In the above technical solution, in step 5, the freeze-drying comprises the following steps, preparing liquid and filling at 20-30 , putting a sample into a freeze dryer, quickly reducing the temperature of a plate layer to-40 , preserving heat for 1h, when the temperature of a partition plate is-10 +/-2 , preserving heat for 1h, quickly reducing the temperature of the plate layer to-40 within 1h, preserving heat for 3h, transferring to a cold trap at-50 , vacuumizing to below 10Pa, firstly preserving the temperature of the plate layer at-5 for 3h, then heating the plate layer to 10 within 3h, when a product is at 0 , starting to increase the temperature of the plate layer to 40 , and when the product is increased to 30 , preserving heat for 5 h

There is no strict cycling requirement, but it is advisable to take occasional breaks to assess its ongoing necessity

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Remember, the goal of any weight loss program is to feel better, not worse