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For actin and Drebrin immunoblots, proteins were prepared in NuPAGE LDS sample buffer (4) (Life Technologies, NP0007), split into reduced (+2% 2-ME) and nonreduced aliquots and heated at 70 C for 10 min 28

However, as Phase 3 trials progress and regulatory submissions become more likely, it is important for patients and clinicians to be prepared for informed conversations about its use

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Lee Transplantation Proceedings.2012