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While our experiments illustrate the ability of IH to negatively regulate Tbr2 + INPs, our data suggest that IH exposure with durations greater than ten days is required to see evidence of pro-neurogenesis signaling within early neuronal progenitor populations

At ~50% confluency, cells were co-transfected with full-length Flag-tagged or MYC-tagged substrate constructs and HA-tagged protease constructs using Lipofectamine 3000

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,,IwamaK,SasakiM,HirabayashiS,OhbaC,IwabuchiEMiyatakeSet al.Milder progressive cerebellar atrophy caused by biallelic SEPSECS mutations.2016;61:527531 103