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elata , from seed germination to flowering plants, can take nearly three years and includes five stages: seed germination, protocorm growth and development, the first asexual reproduction to the formation of an immature tuber, the second asexual reproduction to the formation of a mature tuber, and the bolting, flowering and seed setting of the mature tuber ( Figure 1 )

Immunohistochemistry Formalin-fixed, paraffin-embedded tissue sections (5 m) were used for haematoxylin and eosin staining and immunohistochemical analyses

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