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Before each assay appropriate amount of aliquots were thawed, suspended (1 10 5 cells/ml) in assay medium (DMEM w/o phenol red, 10 mM Hepes, 2% ovalbumin and 0.2% Pluronic F-68) and then transferred into a 96 well plate (5 10 3 cells/50 l) followed by addition of ligand and/or Fab 3F52 diluted in assay medium (50 l/well)
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373 with calorie restriction)
Recruitment of cBAF and PBAF complexes is required for transcription of SLC7A11 SLC7A11 To determine the mechanism underlying the attenuation of SLC7A11 mRNA expression in SWI/SNF-deficient cancer cells, we investigated the involvement of SMARCA4 (a component of cBAF, PBAF, and ncBAF) and SMARCB1 (a component of cBAF and PBAF) in transcriptional upregulation of SLC7A11
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