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Generation of CRISPR KO strains To generate a knockout of glutathione reductase (GSR) in HEK293 cells, a guide RNA sequence targeting exon 1 was cloned into the pSpCas9(BB)-2A-GFP (PX458) vector, gifted from Feng Zhang (Addgene plasmid #48138, Watertown, MA, United States) 84
The sections below walk through what published and preclinical research actually documents mechanism, timeline, outcome areas, and known limitations in the data without extrapolating into personal-use guidance
10.1038/sigtrans.2017.23 63 LiuS.JinX.GeY.DongJ.LiuX.PeiX.et al (2025)
10, Supplementary Data 5)
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