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Alternatively, Acetyl-L-Carnitine can be taken on its own, mixed with water or a beverage of choice, preferably on an empty stomach or before exercise

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An aliquot of Krebs buffer (50 l) was mixed with an equal volume of 0.2 N HCl in an uncapped PCR tube, which was then transferred into a 1.5-ml Eppendorf tube containing 0.5 ml of unlabeled distill water