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Mannik M
Briefly, 1 mg of protein was added on the IPG strip (pH 47, 24 cm), and the pre-programmed power supply (EPS 3500l, Amersham Pharmacia Biotech) was started immediately
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Ensure you understand the plan at each stage: ask your doctor How long do we anticipate Ill need these? at the outset, and at follow-ups ask Are we going to recheck levels or try stopping soon? This will give you a sense of the expected duration
There's no evidence that you build tolerance to BPC-157, but cycling off periodically is still considered good practice
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