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Briefly, ~0.4 kb DNA upstream (5 flank) of the open reading frame (ORF) was amplified by PCR of genomic DNA using primers P1 and P2 (Supplementary Table 1 and Supplementary Fig

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To further mitigate risk, many studies involve sentinel subjects typically only 1 participant on the active drug and 1 on placebo who receives the investigational drug before the remaining participants in the cohort are dosed

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