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Holotoxin delivery and cell viability (CellTiter-Glo assay) 6His-tagged proteases fused to the BoNT translocation domain via a thrombin-cleavable linker were expressed and purified as described above

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Vitamin B-12 is essential for the creation of DNA and the metabolism of cells, and deficiencies in these can lead to anemia, fatigue, muscle weakness, digestive issues, nerve damage, and mood swings