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The time schedule of the device for amplification included three stages: 1- a cycle of initial denaturation at 95C for 5 min 2- forty cycles of amplification at 95C for 15 s, 60C for 15 - 20 s, and 72C for 15 - 30 s 3- a melting curve cycle at 95C for 15 s and 65C for 60 s The relative expression of the desired genes (Table 2) was performed using the CT method proposed by Livak and Schmittgen (24)

This improvement is achieved by decreasing oxidative stress, as evidenced by reduced levels of malondialdehyde (MDA) and increased levels of the antioxidant enzymes superoxide dismutase (SOD) and glutathione peroxidase (GPx)

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In the context of simultaneous GLP-1 activation and caloric restriction, GIP might help mobilize fat stores more effectively or prevent metabolic adaptations that normally fight weight loss

No approved dosing guidance currently exists