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Five microliters of cell concentrations (10 5 , 10 4 , 10 3 and 10 2 ) of each yeast culture were spotted onto YPD plates treated or not with H 2 O 2 4 mM, in presence or in absence of the GSH precursors (0,02 M glutamate, 0,02 M cysteine, 0,02 M, glycine) or with BSO (L- buthionine-sulfoximine) 5 mM

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PMID 9539826 Bowers CY, et al

While freezing did not impact the concentrations of NAD + , NADH, GSH, or GSSG, it triggered the conversion of NADPH to NADP+, with about 508% of NADP + originating from oxidized NADPH (Supplementary Figure 1m-r)

FASEB J (2015) 29(12):476671