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Cell proliferation was evaluated using a Promega CellTiter 96 AQueous One Solution Cell Proliferation Assay (MTS) as per the manufacturers instructions (Promega G3580)

Dosing tiers: Topical application (creams containing 1-3% GHK-Cu) targets skin-specific aging: wrinkles, laxity, and hyperpigmentation

Months 4 to 6: meaningful visual comparison becomes possible

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