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Previously, Jaanson et al ., (2014) developed a similar screening assay in stable HeLa cell 34

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TC2 is degraded within a lysosome, and free B 12 is released into the cytoplasm, where it is transformed into the bioactive coenzyme by cellular enzymes

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It was first described by Raun and colleagues in 1998 and studied in the laboratory for the ghrelin-receptor pathway