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In brief, a fixed volume of plasma or serum (50 L) was spiked with internal standard

Its core process involves multiple key steps and enzymatic reactions: Fatty acid activation and acy l-carnitine formation Fatty acid activation: Fatty acids are first catalyzed by acyl-CoA synthetase in the cytoplasm and combine with CoA (CoA) to form acyl-CoA (Acyl-COA)

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