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Abbreviations BAK: Benzalkonium chloride CCK-8: Cell Counting Kit-8 CHCl3: Chloroform CLSM: Confocal Laser Scanning Microscope CSR: Cell Survival Rate DED: Dry Eye Disease DPPC: 1,2-Dipalmitoyl-Snglycero-3-Phosphocholine DSPE-mPEG2000: 1,2-stearoyl-sn-glycerol-3-Phosphoethanolamine-N-[methoxy (polyethylene glycol)-2000 DCFH-DA: 2,7-Dichlorodihydrofluorescein Diacetate DiI: 1,1-dioctadecyl-3,3,3,3-tetramethylindocarbocyanine perchlorate DAPI: 4,6-Diamidino-2-Phenylindole DLS: Dynamic Light Scattering DL: Drug-loading EE: Encapsulation efficiency FH: Fumarate hydratase GCs: Glucocorticoids GLU: Glutamate GSH: Glutathione GPX4: Glutathione peroxidase Hyd: Hydride HCEC: Human Corneal Epithelial Cell HOS: Hyperosmolar stress H&E: Hematoxylin and Eosin INS: Insulin IGFBP-3: Insulin-like Growth Factor Binding Protein-3 INSR: Insulin Receptors IGF-1R: Insulin-like growth factor 1 receptor IGF-1: Insulin-like growth factor 1 IOP: Intraocular pressure MPTP: Mitochondrial Permeability Transition Pore MRM: Multiple Reaction Monitoring MS: Mass Spectrometry M: Mean NPs: Nanoparticles NaCL: Sodium Chloride OS: Oxidative Stress OCT: Optical coherence tomography OPLS-DA: Orthogonal projections to latent structures- discriminant analysis PEI: Polyethyleneimine PVDF: Polyvinylidene Fluoride QoL: Quality of Life ROS: Reactive Oxygen Species SDS-PAGE: Sulphate-Polyacrylamide Gel Electrophoresis SD: Standard Deviation TCA: Tricarboxylic Acid tBUT: tear Break Up Time TUNEL: Terminal Deoxynucleotidyl Transferase-mediated Nick End Labeling UHPLC: Ultra-High Performance Liquid Chromatography References Clayton JAD, Eye

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Role of branched-chain amino acid metabolism in the pathogenesis of obesity and type 2 diabetes-related metabolic disturbances BCAA metabolism in type 2 diabetes

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