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RT-PCR An RNeasy kit (Qiagen, 74104) was used to extract total RNA from mice hippocampal tissue, and then the Transcriptor High Fidelity cDNA synthesis kit (Roche, 5081963001) was used to reverse transcribe the RNA into cDNA
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Variants in your GLP1R gene (rs6923761), GIPR gene (rs1800437), and FTO gene (rs9939609) influence how strongly you respond to GLP-1 and how fast appetite returns after stopping
Step 3 Before injecting semaglutide, clean the injection site with an alcohol swab and wait for it to dry completely
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