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After undergoing two washes with PBS, the cells were serum-deprived for 1 h in KrebsRinger bicarbonate HEPES (KRBH) buffer including 1% bovine serum albumin (BSA), followed by KRBH buffer containing 1% BSA and 11.1 mM glucose for 0.5 h
These numbers come from pharmacokinetic models, not clinical measurements
It was observed that CCR10 expression decreased ( Fig
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