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After 3 h, the medium was removed and replaced with medium as follows: HUVEC and HDMEC were cultured in endothelial cell basal medium supplemented with 2% (HUVEC) or 1% (HDMEC) FCS, and 90 g ml 1 heparin, with or without hHGF or dimeric macrocycle
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The cells were incubated at room temperature for 30 minutes in the dark, and the cell cycle was analyzed by flow cytometry (Win Bryte HS cytometer [Bio-Rad], using software Win Bryte, Bio-Rad Laboratories Inc., Hercules, CA, USA)
The studies are still being conducted
