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For co-culture experiments, Caco-2 cells were cultured with growth media without antibiotics and were seeded on a 24-well plate (at 2 10 5 cells per well)
some respond strongly at lower doses, while others report minimal benefit
Then use a fresh insulin syringe (29-31 gauge) for each dose
Do not skip these
Rapid entry into the bloodstream Once injected, vitamin B12 enters the bloodstream quickly
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