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Tissue was homogenized by probe sonication (2 10 pulses, 40% power output), and particulate matter was removed by passing samples through 0.4 m syringe filters
Thus, HCC cells can internalize extracellular proteins by increasing the expression of a membrane ruffling protein called EH domain-containing protein 2, leading to resistance to glutamine deprivation under hypoxic conditions 122
Genome Res (2009) 19:231723
These studies highlight how complex the interaction between the endocannabinoid system and microglia is
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