Materials and methods 2.1 Bacterial strains and growth conditions The mutagenesis procedure [17], DNA transformation procedures [18], growth conditions [19], and stock preparation of the pneumococcal strains [20] have been previously reported
Pallichankandy S, Thayyullathil F, Cheratta AR, Subburayan K, Alakkal A, Sultana M, et al
The cryo-EM grid was prepared by applying 3 l of the protein samples to glow-discharged Quantifoil R1.2/1.3 300-mesh gold holey carbon grids (Quantifoil, Micro Tools GmbH, Germany)
Protein-Packed Sources 6
The liquid delivery format provides convenient daily performance and wellness support
(21) The discrepancy between the clinical trials by Iepson et al