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those involving the -glutamyl portion of GSH, mediated by -glutamyl transpeptidases, with the rest involving the sulfhydryl moiety, which is responsible for the redox and nucleophilic reactions
The LC-MS/MS was performed on both polar and hydrophobic metabolites using an Ultimate 3000 RSLC nano coupled to an HPG pump or a Vanquish Flex UPLCs coupled to Orbitrap Fusion Lumos Tribrid mass spectrometer (Thermo Fisher Scientific) in HILIC LC-MS and RP-LC-MS, operated in positive and negative ionization modes as described previously 30
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Injections may be sufficient for maintenance or targeted support, while IV therapy is often chosen for more comprehensive antioxidant or detox focused goals
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