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This QC sample was plated at different positions on the 96-well plate and injected six times to calculate the coefficient of variation (CV) for data quality control, and undetectable metabolites were excluded from the analysis

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Injection-Site Reactions and Product-Quality Concerns Any injection-based administration route can introduce risks such as pain, irritation, infection, contamination, or improper handling when sterile technique and regulated manufacturing controls are absent 18

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