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Maintenance doses are typically given monthly, but some people may require injections every few months

After staining, sections undergo a series of alcohol dehydrations, clearing in xylene, and are then mounted with a neutral resin before being examined under a microscope (BDS400-FL, Ote, Chongqing) for histological evaluation

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This step ensures that the needle remains sterile as it draws the B12 solution from the vial, preventing any contamination of the medicine