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Curr Diab Rep 18(11):123124

This is closed to data showing that HFD enhances intestinal stemness 50 and that calorie restriction can affect stem cell activity by augmenting the function of Paneth cells, which indirectly increases the number of intestinal crypt base columnar stem cells 50

Baseline measurements were taken after an initial 5 min incubation with coelenterazine-h or NanoGlo-containing HBSS, after which cells were treated with either a vehicle (PBS) or the respective ligands

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These combined effects support applications in regenerative medicine, anti-aging research, and cellular repair studies