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Others have used a similar approach to understand how cell development and health are affected by insulin mutations or SNPs derived from monogenic patients, neonatal diabetes patients, and GWAS studies (228238)

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siRNA knockdown and plasmid overexpression For suspension cells, 1 10^6 cells were washed with OPTI-MEM (Gibco, USA) to remove serum, resuspended in OPTI-MEM containing 50 nM siRNA (RiboBio, China) or 3 g/L plasmid (GeneRulor, China), and then transferred to an electrode cuvette

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