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The authors of a 2022 review described the relationship as a vicious cycle: melatonin synthesis decreases as you grow older, and the aging process worsens because of melatonin deficiency
Neurosci Bull 33:183193
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For compound testing, an ECHO 550 acoustic liquid-handling system (LabCyte), was used to dispense testing compound DMSO stock into assay plate, giving a final assay top concentration of 100 M and 1% DMSO
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