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Common side effects can include mild irritation at the injection site or temporary symptoms such as nausea, fatigue, or headaches

They did not irritate the skin at all

The tissue surrounding the injection point, which maintains a tightly regulated pH environment, responds to this disruption with the brief inflammatory signals that produce the stinging or burning sensation

Peer review Peer review information Nature Neuroscience thanks Stig Cramer and the other, anonymous, reviewer(s) for their contribution to the peer review of this work

Subcutaneous technique is straightforward: clean the site, pinch skin, insert needle at 45-degree angle, inject slowly over 30 seconds, withdraw and dispose

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