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The membrane was first blocked in 5% (wt./vol.) skim milk powder (Lab M Limited, Heywood, UK) in tris-buffered saline with 0.1% tween20 (TBST) for 1 h at RT, then was probed with the primary antibody (concentrations and manufacturers in Supplementary Table 4) diluted in TBST with 5% BSA for 16 h at 4 C on a rocker

B12 is typically administered intramuscularly into the deltoid (upper arm) or the gluteal muscle

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Use the tirzepatide dosage calculator to determine exact volumes based on your specific concentration