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B12 from food and supplements has to pass through a fairly involved process in the stomach and gut before your body can use it, and that process becomes less efficient with age, with certain medications, and with diets that include little or no animal protein

Look for changes in absorption: If you notice a weaker effect, it may have degraded

V., & Ryzhak, G

Glandular lining cells are hyperbasophilic and have large nuclei and nucleoli but occasionally have clear cytoplasm due to accumulated glycogen

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The result is a prolonged half-life of ~13 hours , making it suitable for once-daily injection ( [112] ) ( [113] )