Nevertheless, evidence was obtained that the enzymatic reaction is also required to sustain ascorbate accumulation induced in these conditions (Hamada et al
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Then pellets were resuspended in lysis buffer (Tris-HCl 100 mM and 0.5% Triton X-100, pH 8) containing Halt Protease and Phosphatase Inhibitor cocktail (Thermo Scientific, 1861280), thoroughly vortexed for 1 min and incubated for 15 min on ice
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This includes: Vegans and vegetarians who may not obtain sufficient B12 from their diet Older adults, as the ability to absorb B12 from food decreases with age Individuals with certain medical conditions, such as Crohns disease or celiac disease, that can impair B12 absorption People taking certain medications, such as metformin or proton pump inhibitors, which can interfere with B12 absorption Who Should Consider Getting B Complex Shots
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