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Huh7 and H1299 cells (1 10 4 per well) were sequentially treated with 1.5 M oligomycin, 1 M carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone and 1 M rotenoneantimycin A (both 0.5 M) (all from Sigma)
Protein Sources Certain proteins are rich in amino acids that contribute to glutathione synthesis: Whey Protein : This high-quality protein is a rich source of cysteine, which is vital for glutathione production
Glutathione aids in detoxification by binding to harmful substances and facilitating their elimination from the body, promoting liver health and overall detoxification pathways
SLC31A1 is a copper-specific transport protein located in the parietal membrane of enterocytes
2.3 DSS-induced models and GHK-Cu treatment DSS is extensively utilized in animal models of UC due to its capacity to induce histopathological, clinical, and immunologic responses that closely resemble the manifestations observed in patients with IBD (Eichele and Kharbanda, 2017)