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Head position: Ensure that the subject is in a tilted head position
In these cases, individuals might actually experience weight loss rather than gain
Cytokine analysis Enzyme-linked immunosorbent assay (ELISA) is used to detect the expression levels of cytokine in the serum
After 60 min at room temperature, a microplate reader (Rubystar, BMG) was used to measure the fluorescence transfer at ex = 337 nm and em = 620 and 665 nm
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