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Take a balanced diet and stay in contact with your doctor to manage these effects in order to prevent further complications

Galloway, D.C., et al., Regulation of human gamma-glutamylcysteine synthetase: co-ordinate induction of the catalytic and regulatory subunits in HepG2 cells

After that, samples were loaded with Laemmli Buffer and then boiled for 5 min at 95 C to make sure of protein denaturation (Hirano 2012)