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The authors attribute this shift to an imbalance between endothelium-derived relaxing factors (EDRFs)nitric oxide, prostacyclin, endothelium-derived hyperpolarizing factors, and natriuretic peptidesand endothelium-derived contracting factors (EDCFs), including endothelin-1, thromboxane A2, reactive oxygen species, and Rho-kinase signaling

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Results Human GLP-1R -cell interactome reveals binding of agonist-stimulated GLP-1R to signalling effectors, with strong prevalence of ERMCSs-localised proteins To gain a comprehensive understanding of the specific downstream partners and signalling mediators engaged by active GLP-1Rs in pancreatic -cells, we performed an interactome analysis of proteins that bind to the receptor under vehicle versus 5-minute stimulation with the GLP-1RA exendin-4 in a subline of rat INS-1 832/3 -cells stably expressing SNAP/FLAG-tagged human GLP-1R (SNAP/FLAG-hGLP-1R)