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A 20-ml volume of the starter culture was inoculated into 1 l of medium and incubated at 37 C until an optical density of 0.40.8 was reached
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This causes problems, as not enough GHK is available to provide the health benefits it gives
(PubMed) Clark WM, Wechsler LR, Sabounjian LA, Schwiderski UE, Citicoline Stroke Study Group