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For instance, to induce different degrees of starvation, the culture medium of HUVECs was changed at 48, 72, or 96 h, representing different degrees of starvation in comparison with non-starved cells (medium changed every 24 h)

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J Sci Med Sport 19(3):200204 Kikuchi N, Yoshioka T, Taniguchi Y, Sugaya H, Arai N, Kanamori A, Yamazaki M (2019) Optimization of leukocyte-poor platelet-rich plasma preparation: a validation study of leukocyte-poor platelet-rich plasma obtained using different preparer, storage, and activation methods