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glabrata was performed using the lithium acetate protocol, as described previously ( pCgACT-CgATG1, in which CgATG1 was expressed under the control of the CgATG1 native promoter, was constructed as follows: a 3,781-bp DNA fragment containing the CgATG1 promoter, ORF, and 3-UTR was amplified using CgATG1-F(-596FL)-Sal and CgATG1-R(+356FL)-Kpn, digested with SalI and KpnI, and inserted into the SalI-KpnI site of pCgACT ( CgATG1 -reconstituted strain and its control strains were constructed by transformation of the Cgatg1 strain with pCgACT-CgATG1 and pCgACT, respectively
10.1002/tox.22944 152 MaZ.KhalidN.ShuG.ZhaoY.KobayashiI.NevesM
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This water contains 0.9% benzyl alcohol as an antimicrobial preservative, allowing the reconstituted solution to remain stable for several weeks when refrigerated
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