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Ratiometric image analysis was conducted using a Python script (available from The background was subtracted for each channel and the ROIs (=transfected cells) were determined by thresholding or with the Cellpose 40 algorithm in the channel of the FP
Precipitated fungal RNA was collected by centrifugation at 20,000 g at 4 C for 15 min
doi: 10.3233/JAD-190198
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