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Pro-inflammatory actions of red blood cell-derived DAMPs
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Peptides were required to be at least 5 amino acids long and fully tryptic
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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine
Using a 1:100 ratio of mg tissue to L KME buffer (20 mM MOPS, 120 mM KCl, and 1 mM EGTA, pH 7.4), tissue was homogenized in 2 s bursts separated by a 2 s rest for a total of 10 s (or 3 cycles) at 16,600 rpm using IKA T25 digital Ultra-Turrax homogenizer
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