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Seed invigoration with water, ascorbic and salicylic acid stimulates development and biochemical characters of okra ( Ablemoschus esculentus ) under normal and saline conditions

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The RTqPCR mixture consisted of 5 L of SYBR Green Master Mix (Yeasen, Shanghai, China), 1 L of upstream primer, 1 L of downstream primer (with a final concentration of 0.2 M for each primer), and 2 L of cDNA, with enzyme-free water added to a final volume of 10 L

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Losing muscle during weight loss is harmful to long-term weight maintenance since muscle is what burns calories